Description: This course provides detailed lectures on Biotechnology, covering concepts like microscopy, autoradiography, etc.
-
Analytical Technologies in Biotechnology
- Basic concepts in microscopy 1
- Basic concepts in microscopy 2
- Dark-field and phase contrast microscopy
- Differential interference contrast and polarization
- Fluorescence and confocal microscopy
- Transmission electron microscopy
- Transmission electron microscopy cont. and scanning electron microscopy
- Basic concepts 1
- Basic concepts 2
- GM counting and Scintillation counting
- Scintillation counting continued
- Autoradiography and RIA
- Safety aspects and applications
- Introduction and Basic concepts in chromatography 1
- Basic concepts in chromatography 2
- Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography (HPLC)
- Ion-exchange chromatography
- Gel-filtration chromatography
- Affinity chromatography
- Gas-liquid chromatography
- Basic concepts in electrophoresis
- Horizontal and vertical gel electrophoresis
- Native gel electrophoresis and SDS-PAGE
- Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods
- Electrophoresis of nucleic acids
- Immunoelectrophoresis and capillary electrophoresis
- Introduction and basic concepts 1
- Basic concepts-2
- Types of centrifuges and analytical ultracentrifugation method
- Separation methods in preparative ultracentrifuges
- Types of rotors
- Types of rotors cont. and care of rotors
- Introduction and basic concepts
- UV-Visible spectroscopy
- Infrared and fluorescence spectroscopy
- Circular dichroism (CD) spectroscopy
- Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography
- Atomic spectroscopy and mass spectrometry
- Polymerase chain reaction(PCR)
- DNA sequencing methods
- Enzyme linked immunosorbent assay (ELISA)
